Investigation of Changes in Liver Microanatomy in the Steatosis Model Created by Permanent Canula in Rats

Objective: The knowledge of nonalcoholic fatty liver disease (NAFLD) and Nonalcoholic Steatohepatitis (NASH) is limited to the findings from available suitable models for this disease. A number rodent have been described in which relevant pathology develops an appropriate metabolic context. In experimental study, it was aimed create a new fat model by giving portal vein rats visualize changes with advanced microscopic techniques. 
 Methods: 28 female were used study. Permanent intraabdominal cannulas inserted into rats. Rats randomly divided four group. Intralipid 20% substance injected through cannula groups during test period. Control group received saline at same rate. At end experiment, animals visualized laser speckle microscope livers sections according stereological method. painted Hematoxylin-Eosin, Oil red o, Masson trichoma, Bodipy, Nile red. Sections evaluated under microscope. Results: Ballooning, inflammation fibrosis observed 2 week intralipid 1 group, rate parenchyma decreased while sinusoid increased, increased significantly (p˂0.05). Conclusion: According findings, steatohepatitis detected intralipid, whereas only steatosis intralipid. Thus, concluded that newly formed rat causes steatosis.